Table 2 Nucleotide sequences of primers used for PCR and construction of Ub::hGH fusion gene
From: Use of Ubp1 protease analog to produce recombinant human growth hormone in Escherichia coli
Name of primer | DNA sequence (restriction sites underlined) | T m(°C) | Restriction site |
|---|---|---|---|
hGHubF | GGGGCCGCGGTTCCCAACCATTCCCTTAAGTAGGC | 57 | R.SacII |
hGHubR | GGGGGTCGACTTAGAAGCCACAGCTGCCCTCC | 58 | R.SalI |