Figure 6

SDS-PAGE assessment of the stability of the pIGDMKUH plasmid in E. coli DH5α cells. Every passage was cultured for 18 h. M, low molecular weight standard (94, 67, 43, 30, 20.1, and 14.4 kDa); 1, 1^st passage of culture with antibiotic pressure; 2, 2^nd passage of culture with antibiotic pressure; 3, 3^rd passage of culture with antibiotic pressure; 4, 4^th passage of culture with antibiotic pressure; 5, purified Ub::hGH as control; 6, 1^st passage of culture without antibiotic pressure; 7, 2^nd passage of culture without antibiotic pressure; 8, 3^rd passage of culture without antibiotic pressure; 9, 4^th passage of culture without antibiotic pressure.