L-lactic acid production from D-xylose with Candida sonorensis expressing a heterologous lactate dehydrogenase encoding gene

Table 5 L – lactic acid production from xylose with various yeast strains

Strain	Xylose (g l ⁻¹ )	Cultivation time (h)	Lactic acid (g l ⁻¹ )	Lactic acid production rate (g h ⁻¹ l ⁻¹ )	Lactic acid yield (g g ⁻¹ )	Xylitol (g l ⁻¹ )	Genetic modifications	Reference
Candida sonorensis	50	168	26.7	0.16	0.53	0*	Lactobacillus helveticus ldhL	This study
Rhizopus oryzae CBS 147.22	59	190	15.2	0.08	0.38	2.0	Wild type	10
Scheffersomyces stipitis	50	72	31.0	0.43	0.60	≤ 1.3	L. helveticus ldhL	11
S. stipitis	100	147	58.0	0.39	0.58	≤ 1.3	L. helveticus ldhL	11
Candida utilis	100	75	2.9	0.04	0.03	1.2	PDC-, 2 x Bos taurus ldhL	12
C. utilis	100	75	43.8	0.58	0.44	25.4	PDC-, 2 x B. taurus ldhL, Candida shehatae XR (NADPH preferring), C. shehatae XDH (NAD⁺ dependent), S. stipitis XK	12
C. utilis	100	75	67.2	0.93	0.67	3.2	PDC-, 2 x B. taurus ldhL, C. shehatae XR (NADH preferring), C. shehatae XDH (NAD⁺ dependent), S. stipitis XK	12

L-Lactic acid production titre, rate and yield with various yeast or fungal strains in buffered minimal xylose medium. Initial xylose and xylitol amounts at the end of cultivations are also indicated. Genetic modifications (ldhL = L-lactate dehydrogenase, XR = xylose reductase, XDH = xylitol dehydrogenase, XK = xylulokinase) have been indicated. *All xylitol had been consumed at the end of cultivation, but in maximum 4.6 g l⁻¹ xylitol had been produced.

ISSN: 1475-2859