L-lactic acid production from D-xylose with Candida sonorensis expressing a heterologous lactate dehydrogenase encoding gene

Table 4 Xylose reductase and xylitol dehydrogenase activities in xyl1Δ and xyl2Δ strains

Strains	Genotype	Xylose reductase activity	Xylitol dehydrogenase activity
Strains	Genotype	mU [mg protein] ⁻¹	mU [mg protein] ⁻¹
wild type		75 ± 1.6	571 ± 21
C689, C690	xyl1Δ	2 ± 0.3	625 ± 47
C684, C685, C686	xyl2Δ	74 ± 0.9	126 ± 4

NADPH-dependent xylose reductase and xylitol dehydrogenase activities in C. sonorensis strains in which the XYL1 or XYL2 gene were disrupted. Wild type C. sonorensis, xyl1Δ strains (C689 and C690) and xyl2Δ strains (C684, C685 and C686) were grown in YNB with 50 g l⁻¹ D-xylose for 24 h. Results are mean ± sem for duplicate activity measurements of wild type strain, two xyl1Δ strains or three xyl2Δ strains.

ISSN: 1475-2859