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Table 2 Effect of overexpressing pentose phosphate pathway genes on riboflavin production and enzyme activities

From: Metabolic engineering of Escherichia coli for the production of riboflavin

Strain/plasmid OD600 a Riboflavin (mg/L) Riboflavin yield (mg/g) b Enzyme activity (nmol/min/mg protein) c
G6PDH d 6PGDH d
RF01S/p15Trc 3.8±0.1 192.1±7.3 23.0±0.9 111.5±11.5 119.1±8.4
RF01S/p15Trc-zg 3.0±0.1 197.4±9.2 24.2±1.4 203.6±18.5 174.7±14.2
RF01S/p15Trc-zgp 3.1±0.1 228.4±0.9 31.9±1.3 199.0±20.0 177.1±17.6
  1. aOD600 is the final optical density of the culture.
  2. bmg/g: mg/g-consumed glucose.
  3. Batch cultures of recombinant strains were carried out in LB medium containing 10 g/L glucose at 37°C as described in methods. Standard deviations were calculated from the results of three independent experiments.
  4. cOne unit of the enzyme activity was defined as the amount catalyzing the formation of 1 nmol of NADPH per min. Results are the mean values of three measurements.
  5. d6PGDH, 6-phosphogluconate dehydrogenase; G6PDH, glucose 6-phosphate dehydrogenase.