Table 1 Bacterial strains and plasmids used in this study
Strain or plasmid | Description | Reference |
|---|---|---|
Strains | ||
E.coli DH5α | supE44 Δ lacU169 (φ80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relAI | [27] |
E. coli DH5a (pUC-gtf) | E. coli DH5a clone with gtf | This study |
E. coli ET12567 | Methylation-deficient E. coli dam-dcm-hsdM | [29] |
Streptomyces sp. 139 | Ebosin producing strain | Lab stock |
Streptomyces sp. 139 (ste7 -) | The gene ste7 disruption mutant | This study |
Streptomyces sp. 139 (gtf) | Ams, thior, the heterologous gene replacement strain of Streptomyces sp.139 | This study |
Streptococcus thermophilus | Wild type | CICC 20370 |
Plasmids | ||
pUC18 | Ampr, E.coli general cloning vector with multiple cloning site | [27] |
pUC-gtf | Ampr, pUC18 derived plasmid carrying gtf gene | This study |
pKC7 | Kmr, Amr, pKC1139 derived plasmid carrying F1, F2 | This study |
pKC7-gtf | pKC7 carrying gtf gene | This study |
pEGM-T-tsr | pEGM-T carrying thio r gene | Lab stock |