Table 3 Bacterial strains and plasmids used in this study

Strains

B. subtilis 168

Wide-type strain, trpC2

Lab Stock

E. coli DH5α

Standard cloning strain

Invitrogen

BS77

B. subtilis 168Δupp ribC* ribO* yhcF* yvrH* ywaA*

This study

BS89

BS77, P ₄₃ ::ribA

This study

BS93

BS77, P ₄₃ ::ribA P ₄₃ ::ribG ΔribP1 ΔribO

This study

BS96

BS77, P ₄₃ ::ribA ΔribO

This study

BS102

BS77, P ₄₃ ::ribA ΔpurR

This study

BS103

BS77, P ₄₃ ::ribA ΔpurR P _pur (-10*)

This study

BS104

BS77, P ₄₃ ::ribA ΔpurR Δatt

This study

BS106

BS77, P ₄₃ ::ribA ΔpurR Δatt P ₄₃ ::purF

This study

BS107

BS77, P ₄₃ ::ribA ΔpurR Δatt P _pur (-10*)

This study

BS110

BS77, P ₄₃ ::ribA ΔpurR Δatt P ₄₃ ::purF ^{D293V K316Q S400W}

This study

BS111

BS77, P ₄₃ ::ribA ΔpurR Δatt P ₄₃ ::purF ^{K326Q P410W(E. coli)}

This study

Plasmids

pUC18

Amp^r

Lab Stock

pC194

Cm^r, Bacillus cloning vector

Lab Stock

pSS

Amp^r, Cm^r, pUC18 containing cat-upp cassette

[36]

pSS-P₄₃-ribA-FB

Amp^r, Cm^r, containing ribA upstream and downstream flanks under P ₄₃ promoter

This study

pSS-P₄₃-ribG-FB

Amp^r, Cm^r, containing ribG upstream and downstream flanks under P ₄₃ promoter

This study

pSS-ribO-FB

Amp^r, Cm^r, containing ribO flanks

This study

pSS-purR-FB

Amp^r, Cm^r, containing purR flanks

This study

pSS-(-10*)-FB

Amp^r, Cm^r, containing Ppur(-10*) mutant flanks

This study

pSS-att-FB

Amp^r, Cm^r, containing att flanks

This study

pSS-P₄₃-purF-FB

Amp^r, Cm^r, containing purF (from B. subtilis) upstream and downstream flanks under P ₄₃ promoter

This study

pSS-purF*(bsu)-FB

Amp^r, Cm^r, containing purF mutant (from B. subtilis) flanks

This study

pSS-purF*(eco)-FB

Amp^r, Cm^r, containing purF mutant (from E.coli) upstream and downstream flanks under P ₄₃ promoter

This study