Figure 2

Molecular characterization of transgenic microalgae. A) Transgenic lines selected in liquid medium supplemented with chloramphenicol were screened by PCR using primers for CAT. A 0.7-kb CAT band was present in the transgenic lines (lane 3,4,6,7) while absent in the wild type (lane 1,2). Lane 5: 125 bp DNA ladder marker. B) Transcript abundance of PtPDK in the transgenic lines determined by qPCR. The relative expression of PtPDK was calculated from the Ct values of PtPDK mRNA using the standard curve method. Results were normalized against the β-actin control gene. C) Enzyme activity of PDK in transgenic and wild type microalgae. All assays were done in triplicates and error bars represent standard deviation.