Production of the short peptide surfactant DAMP4 from glucose or sucrose in high cell density cultures of Escherichia coli BL21(DE3)

Table 2 **Rates of consumption/production for main cultivation parameters in Batch or Fed-batch cultivation (expressed as [mmol-C/gDCW*h] unless otherwise stated)**

Name	Substrate	Phase	SUR	DCW	CO₂	Ac	Fo	D4	Balance [%]
▪ Batch
LBG	LB + glucose	G	48.3 ± 1.3	27.5 ± 3.6	23.5 ± 2.2	ND	ND	0.0 ± 0	ND
		P	23.2 ± 1.1	16.3 ± 2.8	15.9 ± 3.1	3.6 ± 0.3	ND	2.6 ± 0.3	ND
G_m	Glucose	G	50.2 ± 0.9	24.2 ± 0.3	23.8 ± 2.1	0.6 ± 0.0	1.9 ± 0.1	0.0 ± 0.0	99.3
		P	25.2 ± 0.6	8.9 ± 0.4	13.2 ± 0.5	1.9 ± 0.2	0.0 ± 0.0	2.2 ± 0.1	96.1
S_m	Sucrose	G	54.6 ± 0.6	24.3 ± 0.5	23.6 ± 0.1	1.9 ± 0.5	0.4 ± 0.1	0.0 ± 0.0	108.9
		P	28.2 ± 0.4	10.0 ± 1.5	14.6 ± 0.3	2.9 ± 0.1	0.6 ± 0.1	2.1 ± 0.1	93.2
▪ Fed Batch
G_f	Glucose	G	54.3 ± 0.8	23.3 ± 0.3	24.1 ± 1.4	0.6 ± 0.1	2.0 ± 0.4	0.0 ± 0.0	108.7
		F	22.5 ± 0.9	9.8 ± 0.2	11.0 ± 0.6	0.0 ± 0.0	0.1 ± 0.0	0.0 ± 0.0	107.5
		P	4.0 ± 0.2	1.2 ± 0.1	2.0 ± 0.3	0.1 ± 0.0	0.0 ± 0.0	0.4 ± 0.0	107.5
S_f	Sucrose	G	57.9 ± 1.5	25.1 ± 0.3	25.6 ± 1.1	0.8 ± 0.2	2.6 ± 0.2	0.0 ± 0.0	106.8
		F	24.9 ± 0.6	11.1 ± 0.1	13.2 ± 0.2	0.0 ± 0.0	0.1 ± 0.0	0.0 ± 0.0	102.3
		P	4.6 ± 0.5	1.3 ± 0.4	2.7 ± 0.6	0.1 ± 0.0	0.0 ± 0.0	0.4 ± 0.0	104.1

Phase connotes batch Growth phase (G), growth during Feeding (F = 5.5 mL/h for fed-batch) or Production (P) phases (the period between induction and the peak product accumulation); GR, Growth Rate; SUR, Substrate Uptake Rate; CO ₂, carbon dioxide; Ac, acetate; Fo, formate; D4, recombinant DAMP4; Balance, percentage ratio between substrate consumption and product formation rates. ND: Not Determined. Experimental error expressed as SD, n ≥ 2.

ISSN: 1475-2859