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Table 1 the concentrations of key metabolites involved in glycolysis, citrate cycle, pentose phosphate pathway and spinosad synthesis under the control and oxidative condition

From: Suitable extracellular oxidoreduction potential inhibit rex regulation and effect central carbon and energy metabolism in Saccharopolyspora spinosa

Metabolites

72h

96h

120h

144h

168h

Controla

Oxidative

Control

Oxidative

Control

Oxidative

Control

Oxidative

Control

Oxidative

Glycolysis

Fructose-6-P

1

1

1.13

1.62

0.94

1.35

1.26

0.75

0.67

0.93

glyceraldehyde 3-phosphate

1

1

0.97

1.54

1.00

2.09

0.94

1.21

0.96

0.53

Pyruvate

1

1

1.26

1.56

1.79

1.24

0.81

1.50

1.16

1.38

Acetyl-CoA

1

1

1.31

1.79

1.06

2.53

1.22

0.97

0.52

0.89

L-Lactate

1

1

2.32

0.35

1.39

ND

1.16

0.17

1.63

ND

Pentose phosphate pathway

Glucose-6-P

1

1

1.74

6.20

2.16

7.22

1.92

7.16

1.31

4.97

6-phosphogluconate

1

1

0.73

0.81

0.44

0.53

0.25

0.21

ND

0.14

Citrate cycle

Citrate

1

1

1.29

2.89

1.12

1.96

0.93

1.89

0.77

1.37

Oxaloacetate

1

1

0.59

1.28

0.41

1.05

0.37

0.92

0.46

0.79

Succinyl-CoA

1

1

1.62

3.42

1.73

4.11

1.07

3.21

0.93

3.07

Spinosad synthesis related

Threonine

1

1

1.16

1.39

0.50

0.85

0.26

0.68

ND

0.42

Valine

1

1

1.14

2.69

1.69

3.99

1.92

3.51

0.25

0.73

Isoleucine

1

1

0.51

1.17

0.27

0.86

0.20

0.57

0.26

0.45

Propionyl-CoA

1

1

1.47

2.73

1.94

3.16

1.86

3.37

1.66

2.79

Malonyl-CoA

1

1

1.24

1.99

1.17

1.48

0.97

1.72

1.10

1.91

Methylmalonyl-CoA

1

1

1.05

1.50

1.03

1.44

1.21

1.90

0.88

1.16

  1. a:The concentration at 72h was the set as 1; ND: Under the lower limit of detection.