Table 4 Oligonucleotides used in this study

malE-integr

5′-AAGGTAAACTGGTAATCTGGATTAACGGCGATAAAGGCT ATAACGGTCTCGCTGTCAAGGCGCACTCCCGTTCTGG-3′

malG-integr

5′-GATCGGTAATGCAGACATCACGGCAGCGGCGGCAAAGTC ACCCCACAGGTAGTTTTCAGGGTTATTGTCTCATGAGCG-3′

rbsD-integr

5′-ACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCT GGGACATACCGATATCAAGGCGCACTCCCGTTCTGG-3′

rbsK-integr

5′-ATTCACGCTAGCCCATACACCACGACTTCCTAAAGTAATC AGTACAGTACGGATACCCAGGGTTATTGTCTCATGAGCG-3′

malK-screen

5′-GCTTTCGTTACATTTTGCAGCTGTACG-3′

cat-control

5′-CCGTCACAGGTAGGCGCGCC-3′

glpX-for (NdeI)

5′-TTTTCATATGAGACGAGAACTTGCCATCGAATTTTCC-3′

glpX-rev (HindIII)

5′-TTTTAAGCTTCAATCAGAGGATGTGCACCTGCATTTCC-3′

glpK_for (NdeI)

5′-GCTGTCATATGACTACGGGACAATTAAACATG-3′

glpK_rev (HindIII)

5′-CCGGATGCGGCATAAAAGCTTCATTCGGC-3′

glpKG232D-for

5′-GTATACGGTCAGACTAATATTGGCGACAAAGGCGGCAC-3′

glpKG232D-rev

5′-GTGCCGCCTTTGTCGCCAATATTAGTCTGACCGTATAC-3′