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Figure 4 | Microbial Cell Factories

Figure 4

From: The important ergot alkaloid intermediate chanoclavine-I produced in the yeast Saccharomyces cerevisiae by the combined action of EasC and EasE from Aspergillus japonicus

Figure 4

Roles of EasE signal peptide, and EasC tripeptide ARL. Relative production of Me-DMAT (left panel) and chanoclavine-I (right panel) was analysed, of a Me-DMAT producing strain, co-expressing EasC and EasE proteins with different localization signals. Wild type EasE_Aj was co-expressed in combination with either wt EasC_Aj (A) or EasC_Aj -PTS1which has no PTS1 (B). Removal of the PTS1 tripeptide ARL resulted in a slight increase of Me-DMAT and chanoclavine-I. However, when an N-terminally truncated EasE_Aj –N sig. was co-expressed with either wt EasC_Aj (C) or EasC_Aj -PTS1 (D), production of chanoclavine-I was essentially abolished. The loss of function, due to the N-terminal truncation of EasE_Aj, resulted in increased accumulation of the precursor Me-DMAT (C and D compared to A and B). The vertical axes show arbitrary units based on areas under the HPLC peaks.

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Microbial Cell Factories

ISSN: 1475-2859

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