Table 4 Primers used in the present study (restriction sites are italics)
Primers | Sequence (5’ → 3’) |
|---|---|
For plasmid construction | |
TA-U | CCGGAATTC AAATGAAATAATACGGGAAGAGCG |
TA-D | CGGGGTACC CGGTAGCGATAATGTAAGAGGGTT |
TB-U | ACGCGTCGAC GAACAGAACACAAAAGGAACAC |
TB-D | ACATGCATGC GAACCGCAATATTCAGAAACAC |
SA-U | CCGGAATTC CTTATAACGTGGGCCATGTCAT |
SA-D | CGCGGATCC CTAGTGACGTTGTCGTATTTGG |
SB-U | CGCGGATCC TCAACGAGAAATGTTGTGTAGC |
SB-D | CCCAAGCTT ACATATGCTCATCGGGAAAACC |
Kan-U | CGCGGATCC CAGCTGAAGCTTCGTACGC |
Kan-D | CGCGGATCC GCATAGGCCACTAGTGGATCTG |
For PCR verification | |
YT-U | TCTTGTCTGTCTGCTTCTTCACTGT |
YT-D | AAAGAGTGTGAAGTGACGGCTATG |
YS-U | CACACTCCGTTCTTAGTGGTTGTT |
YS-D | ATCCACCGTAGAACCCAAAGCATT |
K-U | CTTGCTAGGATACAGTTCTCACATCA |
K-D | CGCATCAACCAAACCGTTATTCATTC |
Z-U | CCCACACACCATAGCTTCA |
Z-D | AGCTTGCAAATTAAAGCCTT |