Figure 1From: Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate(A) Schematic representation of the pPIC9mGM-CSF expression vector and (B) Secretion signal processing. The S. cerevisiae α-factor secretion signal is removed by the consecutive action of two enzymes: Kex2p cleaves between the arginine and the glutamic acid residue in the sequence EKREAEA; Ste13p removes both EA repeats.Back to article page