Figure 3

Identification of the secretion pathway. In the CM124 strain Sec mediated secretion is inducible via addition of L-arabinose. Incubation times were kept very short to ensure cell viability of the cell culture without functional Sec pathway. Furthermore, low expression levels of the recombinant protein prevent contamination of the subcellular compartments due to overexpression of the target protein. Therefore, the subcellular localization of N^proEDDIE was determined via immuno blot. GroEL immuno blot was performed to control osmolysis mediated cell lysis. -I: whole cell sample without induction of recombinant protein expression, +I: whole cell samples with induction of recombinant protein expression, P: periplasmic fraction, C: cytoplasmic fraction.