Identification and characterization of a novel fumarase gene by metagenome expression cloning from marine microorganisms

Table 1 Kinetic parameters of fumarases from various sources.

Substrate/Source	Fumarate			L-Malate
	K_m (mM)	V_max (μmol product/min/mg enzyme)	k_cat/ K_m (/mM/s)	K_m (mM)	V_max (μmol product/min/mg enzyme)	k_cat/ K_m (/mM/s)
Pelotomaculum thermopropionicum (MmcBC) [7]	0.43	201	509	0.59	23.7	42.7
Thermus thermophilus AS15-1 (pH7.5) [21]	1.0	1340	NR	NR	NR	NR
Bacterium MPOB (DSM 10017) [24]	0.25	NR	690	2.38	NR	540
Pyrococcus furiosus (Pf fumarase) [25]	0.34	1376	3200	0.41	1892	3700
Marine samples (FumF, this work)	0.48	827	1900	3.15	282	100

ISSN: 1475-2859