Figure 2
From: Improved vanillin production in baker's yeast through in silico design
Comparison of targets predicted by OptGene for improved VG productivity. A - Biomass versus VG yield is represented for each knockout mutant phenotype obtained after OptGene simulation using three different reference flux distributions for MOMA. Experimental yields observed for VG0 are represented by the red empty triangle and bar. B/C/D - The predicted VG yield (mol/molglc) obtained for each knockout mutant after OptGene simulation using Reference 1/2/3 is given by the length of the coloured bars. For each reference flux distribution, the R3 score was estimated for each of the mutants was calculated and normalized to the mutant presenting the highest value: MAmut/MAmax*100. 100% represents the mutant with highest R3 score for a given flux distribution. Candidate 80%PDC is not a knockout in silico mutant, rather its PDC reaction is constrained to 80% of the upper bound.