Expression and purification of ELP-intein-tagged target proteins in high cell density E. coli fermentation

Table 1 Product yields and cell growth in various strains and media recipes

Shake Flask	Strain	Medium	Yield¹β-gal (mg/L)	Final OD₆₀₀
	Rosetta	TB*	106	12.8
	Rosetta	NBS	121.5	6
	Rosetta	NBS (no IPTG)	7.8	16.1
	BLR	TB*	109	9.7
	BLR	NBS	57	3.23
	ER2566	NBS	80	14.6
Batch fermentations	Strain	Medium	Yield ¹ β-gal (mg/L)	Final OD ₆₀₀
	Rosetta	TB* (pH 6.9, dO₂ > 20%)	31	25
	Rosetta	NBS (pH 6.9, dO₂ > 20%)	39	28
	Rosetta	NBS (pH 6.9, dO₂ < 10%)	104	26
Fed-batch fermentations	Strain	Medium	Yield ¹ β-gal (mg/L)	Final OD ₆₀₀
	Rosetta	Feed 1	180	73
	Rosetta	Feed 2	173	128
	Rosetta	Feed 3	127	95
	Rosetta	Feed 4	160	121
	Rosetta	Feed 5	234	151
	Rosetta	Feed 5	236²	129

¹ These yields are based on one round of inverse transition cycling to show overall yield trends. More accurate absolute yield information can be found in Table 2.
²Yield of OPH-S5 (mg/L)

ISSN: 1475-2859