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Figure 7 | Microbial Cell Factories

Figure 7

From: Physiological relation between respiration activity and heterologous expression of selected benzoylformate decarboxylase variants in Escherichia coli

Figure 7

Mechanistic reason for the behaviour of E. coli SG13009 pKK233-2 clones expressing different BFD-variants. (A) BFD-variants with high cofactor binding strength: wild type, His281Ala, Ser181Thr, and partially Leu476Gln. As the thiamine is bound by these BFD-variants with high binding strength, it is not available in the medium and, thus, the growth of these strains is impaired. (B) BFD-variants with low cofactor binding strength: Leu476His, Leu476Pro, Leu476Pro-Ser181Thr.

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Microbial Cell Factories

ISSN: 1475-2859

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