Skip to main content

Table 1 Quantification of allergen expression on the surface by FACS analysis

From: Display of wasp venom allergens on the cell surface of Saccharomyces cerevisiae

 

% of expressing cells

Geometrical mean ratio

Protein

Tryptophan selection

Tryptophan and zeocin selection

Tryptophan selection

Tryptophan and zeocin selection

None

2.4

-

1.0

-

CD20

43.7 ± 1.0

74.9 ± 1.3

3.1 ± 0.2

11.5 ± 0.3

Phospholipase A1 (Ves v 1)

3.3

5.6

1.2

1.1

Hyaluronidase (Ves v 2)

5.1

5.3

1.3

1.5

Antigen 5 (Ves v 5)

32.8

73.4

2.2

9.3

  1. The cells were stained with anti-C-myc antibody conjugated with AlexaFluor488 and analyzed by fluorescent flow cytometry. The intensity of the green fluorescent signal was measured. For calculation of percentage of expressing cells and geometrical mean ratio the labeled cells were compared with non-labeled cells. The labeling of CD-20-expressing cells was performed in triplicates and the average and standard deviation are shown.
Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us