Table 1 Strains and plasmids used in this study.

L. lactis HtrA NZ9000

MG1363 (nisRK genes on the chromosome)

[37]

E. coli TG1

supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5 (rK- mK-) [F' traD36 proAB lacI qZ ΔM15]

ATCC

E. coli DH5α

fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17

Invitrogen

E. coli BL21 (DE3)

F ^- ompT gal dcm lon hsdS _B (r _B ^- m _B ^- ) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])

Novagen

Plasmid

pVE5524

Ery^r, Amp^r; pBS::pIL252::t _trpA ::P59::rbs _usp45 ::sp _Usp45 -nucA-cwa_M6-t1t2

[36]

pVE5523

Ery^r, Amp^r; pBS::pIL252::t _trpA ::P59::rbs _usp45 ::sp _Usp45 -nucA-t1t2

[36]

pSIP502

Ery^r; P _{nisA ::}rbs _{nisA ::}uidA

[66]

pSCNIII

Cm^r

J. Seegers^a

pUC19

Amp^r

[69]

pET28(b)

Km^r

Novagen

pSIPsp-nuc

Ery^r; P _nisA ::rbs_nisA::sp_Usp45-nucA

This Work

pUC104

Amp^r; t _trpA ::P _nisA ::rbs _usp45 ::sp_Usp45-nucA

This Work

pUC104mod

Amp^r; t _trpA ::P59::rbs _usp45 ::sp_Usp45-nucA

This Work

pUC304

Amp^r; t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-nucA

This Work

pUC504

Amp^r; t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45

This Work

pAW004

Ery^r, Amp^r; pBS::pIL252::t _trpA ::P59::rbs _usp45 ::sp_Usp45-nucA-MCS-cwa_M6-t1t2

This Work

pAW005

Ery^r, Amp^r; pBS::pIL252::t _trpA ::P59::rbs _usp45 ::sp_Usp45-nucA-MCS-t1t2

This Work

pAW004Z

Ery^r, Amp^r; pBS::pIL252::t _trpA ::P59::rbs _usp45 ::sp_Usp45-nucA-lacZα-cwa_M6-tlt2

This Work

pAW005Z

Ery^r, Amp^r; pBS::pIL252::t _trpA ::P59::rbs _usp45 ::sp_Usp45-nucA- lacZα-tlt2

This Work

pAW004ZC

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P59::rbs _usp45 ::sp_Usp45-nucA-lacZα-cwa_M6-tlt2

This Work

pAW005ZC

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P59::rbs _usp45 ::sp_Usp45-nucA- lacZα-tlt2

This Work

pGEMc9

Amp^r; pGEMT::with cloned coh9 from cipA

This Work

pGEMc1

Amp^r; pGEMT::with cloned coh1 from cipA

This Work

pGEMc1-c2

Amp^r; pGEMT::with cloned coh1-coh2 from cipA

This Work

pGEMcbm-c3

Amp^r; pGEMT::with cloned cbm3a-coh3 from cipA

This Work

pGEMcbm

Amp^r; pGEMT::with cloned cbm3a from cipA

This Work

pAW104

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _usp45 ::sp_Usp45-nucA-LacZα-cwa_M6-tlt2

This Work

pAW105

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _usp45 ::sp_Usp45-nucA-LacZα-tlt2

This Work

pAW301

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-nucA-cwa_M6-tlt2

This Work

pAW302

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-nucA-tlt2

This Work

pAW304

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-nucA-lacZα-cwa_M6-tlt2

This Work

pAW305

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-nucA-lacZα-tlt2

This Work

pAW307

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-nucA-coh9-cwa_M6-tlt2

This Work

pAW308

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-nucA-coh9-tlt2

This Work

pAW310

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-nucA-coh1-cwa_M6-tlt2

This Work

pAW311

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-nucA-coh1-tlt2

This Work

pAW334

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-nucA-coh1-coh2-cwa_M6-tlt2

This Work

pAW335

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-nucA-coh1-coh2-tlt2

This Work

pAW328

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-nucA-cbm3a-coh3-cwa_M6-tlt2

This Work

pAW329

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-nucA-cbm3a-coh3-tlt2

This Work

pAW331

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-nucA-cbm3a-cwa_M6-tlt2

This Work

pAW332

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-nucA-cbm3a-tlt2

This Work

pAW504

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-lacZα-cwa _M6 -tlt2

This Work

pAW505

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-lacZα-tlt2

This Work

pAW507

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-coh9-cwa_M6-tlt2

This Work

pAW508

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-coh9-tlt2

This Work

pAW510

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-coh1-cwa _M6 -tlt2

This Work

pAW511

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-coh1-tlt2

This Work

pAW534

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-coh1-coh2-cwa_M6-tlt2

This Work

pAW535

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-coh1-coh2-tlt2

This Work

pAW528

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-cbm3a-coh3-cwa_M6-tlt2

This Work

pAW529

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-cbm3a-coh3-tlt2

This Work

pAW531

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-cbm3a-cwa_M6-tlt2

This Work

pAW532

Cm^r, Amp^r; pBS::pIL252::t _trpA ::P _nisA ::rbs _nisA ::sp_Usp45-cbm3a-tlt2

This Work

pETdock1

Kn^r; pET28(b)::with cloned dock1 from celS

This Work

pETUdock1

Kn^r; pET28(b)::PT7::6xHis-uidA-dock1

This Work

pETU

Kn^r; pET28(b)::PT7::6xHis-uidA

This Work