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Table 3 Catalytic activity and protein content of GalOx expressed extracellularly in P. pastoris

From: A comparative summary of expression systems for the recombinant production of galactose oxidase

 

vol. activityb[U·mL-1]

straina

24 h

48 h

72 h

96 h

120 h

120 hc

     

vol. activity [U·mL-1]

protein [mg·mL-1]

spec. activity [U·mg-1]

 

P. pastoris FxE

15.2

29.0

55.0

69.0

73.0

0.28

260

6.1

P. pastoris ExE

15.0

27.0

43.0

60.0

61.0

0.24

254

4.3

P. pastoris FNE

5.3

6.0

6.2

7.6

7.3

0.12

61

1.1

P. pastoris ENE

3.0

5.6

5.7

5.9

6.1

0.10

61

1.0

  1. a See Table 1
  2. b catalytic activity and protein content after incubation with 0.5 mM CuSO4 at 4°C and stirring overnight; wild-type GalOx from F. graminearum and the variant M1 with or without an N-terminal His10-tag were expressed extracellularly in P. pastoris SMD1168H at 25°C for 120 hours
  3. c vol. activity [U·mL-1] before the incubation with 0.5 mM CuSO4
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Microbial Cell Factories

ISSN: 1475-2859

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