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Table 2 Volumetric activity of GalOx expressed intracellularly in P. pastori s

From: A comparative summary of expression systems for the recombinant production of galactose oxidase

 

vol. activityb[U·mL-1]

straina

24 h

48 h

72 h

96 h

120 h

120 hc

P. pastoris FxI

3.2

7.4

11.0

14.0

14.0

0.9

P. pastoris ExI

1.7

4.2

7.1

10.0

11.0

0.8

P. pastoris FNI

0.7

1.3

1.9

2.4

2.5

0.5

P. pastoris ENI

0.6

1.0

1.8

2.2

2.2

0.4

  1. a See Table 1
  2. b vol.activity of GalOx after incubation with 0.5 mM CuSO4 at 4°C and stirring overnight; wild-type GalOx from F. graminearum and the variant M1 with or without an N-terminal His10-tag were expressed intracellularly in P. pastoris GS115 at 25°C for 120 hours
  3. c vol. activity [U·mL-1] before the incubation with 0.5 mM CuSO4
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Microbial Cell Factories

ISSN: 1475-2859

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