Table 1 Expression hosts, galox genes and vectors used in this study

E. coli FNI

E. coli BL21DE3^a

pET16b⁺

Fusarium wild-type

N-terminal His₁₀

intracellular

E. coli ENI

E. coli-optimized

N-terminal His₁₀

P. pastoris FxI

P. pastoris GS115^b

pPIC3.5

Fusarium wild-type

None

intracellular

P. pastoris ExI

E. coli-optimized

None

P. pastoris FNI

Fusarium wild-type

N-terminal His₁₀

P. pastoris ENI

E. coli-optimized

N-terminal His₁₀

P. pastoris FxE

P. pastoris SMD1168H^c

pPICZα-C

Fusarium wild-type

None

extracellular

P. pastoris ExE

E. coli-optimized

None

P. pastoris FNE

Fusarium wild-type

N-terminal His₁₀

P. pastoris ENE

E. coli-optimized

N-terminal His₁₀

P. pastoris PxE

Pichia-optimized

None

P. pastoris PCE

Pichia-optimized

C-terminal His₆