Disruption of reducing pathways is not essential for efficient disulfide bond formation in the cytoplasm of E. coli

Table 3 Vectors reported in this study

Plasmid	Basis	Protein being produced	Co-expression
pFH198	pET23	Mature E. coli DsbC (Asp21-Lys236)	-
pVD157	pET23	S. cerevisiae Erv1p (Met 1-Glu189)	-
pVD158	pET23	S. cerevisiae Erv1p (Met 1-Glu189)	DsbC
pVD80	pET23	MH₆M-mature E. coli PhoA (Arg22-Lys471)	-
pVD82	pET23	MH₆M-mature E. coli PhoA (Arg22-Lys471)	Erv1p
pVD96	pET23	MH₆M-mature E. coli AppA (Gln 23-Leu 432)	-
pFH231	pET23	MH₆M-mature E. coli AppA (Gln 23-Leu 432)	Erv1p
pFH244	pET23	MH₆M-mature E. coli AppA (Gln 23-Leu 432)	DsbC
pFH233	pET23	MH₆M-mature E. coli AppA (Gln 23-Leu 432)	Erv1p + DsbC

Expression from pET23 plasmids is by IPTG induction; all pET23 vectors have a SpeI site added to the multi-cloning site. The co-expressed proteins are either S. cerevisiae Erv1p (Met 1-Glu189) and/or the mature form of E. coli DsbC (Asp21-Lys236).

ISSN: 1475-2859