Figure 5From: Antibiotic-free selection in E. coli: new considerations for optimal design and improved productionConstruction by combined PCR of the 5' ccdA -Kanamycin-3' ccdA fragment. In green the 120 bp sequence identical in 5'ccdA and 3' ccdA fragment and target sites for homologous recombination, in blue the kanamycin resistance gene. Homologous recombination ensures the complete elimination of the Kanamycin resistance gene.Back to article page