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Table 1 Strains and plasmids used in this study.

From: Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into Streptomyces ansochromogenes lead to an improved nikkomycin production

Strains or plasmids Relevant characteristics Sources
Bacterial strains   
   S. ansochromogenes   
7100 Wild-type strain [31]
DNik S. ansochromogenes 7100 containing plasmid pNIK This work
E. coli   
   DH5α F-recA, f80, dlacZ ΔM15 Gibco BRL
   ET12567 (pUZ8002) recE, dam, dcm, hsdS, Cm, Str, Tet, Km [32]
   XL 1-blue MR Δ(mcrA)183(mcrCB-hsdSMR-mrr)173, endA1, supE44, thi-1, recA1, gyrA96, relA1, lac Stratagene
   BW25113 K12 derivative: ΔaraBAD, ΔrhaBAD [33]
Plasmids   
   pBluescript KS- Routine cloning and subcloning vector Stratagene
   pBS Digestion of pBluescript II KS- with BamH I-Pst I, blunt-ended and religation. This work
   supercos 1 Vector for cosmid library construction Stratagene
   pSET152 Integrative vector [22]
   pIJ790 λRED (gam, bet, exo), cat, araC, rep101ts [33]
   cosG4 A cosmid containing most of the nikkomycin biosynthetic genes This work
   pLXP 11 kb DNA fragment containing partial nikkomycin biosynthetic genes inserted into pSET152 [18]
   pNIK A pSET152 based plasmid containing the whole nikkomycin biosynthetic gene cluster This work