PCR analysis of the integration of pNIK into the genome of S. ansochromogenes. (A) M, 100 bp marker; Lane 1, S. ansochromogenes 7100; Lane 2, S. ansochromogenes 7100 (pSET152); Lane 3, S. ansochromogenes DNik. (B) Physical map of pSET152 inserted into the attB site of S. ansochromogenes. Primer test-F and test-R were used to amplify 964 bp DNA fragment from the genomic DNA of S. ansochromogenes 7100 (pSET152) and DNik. Chromosomal DNA is indicated by thin lines, and pSET152 is indicated by thick lines. int, the integrase gene; aac(3)IV, the apramycin resistance gene.