Figure 1

RT-PCR analysis of the mutated promoters (A) and the production of β-Gal driven by these promoters (B, C and D). (A) Real-time PCR analysis of the transcription amount from pLJ-7, pJRINM1, pJRINM2, pJRINM3 and pJRINM4, respectively (16 s rDNA used as the control). (B) The production of β-Gal when cultured in LB. The black square, white triangle, white circle, black diamond and white diamond represents the production of β-Gal from B. subtilis 1A747 harboring pJRINM1, pJRINM2, pJRINM3, pLJ-7 and pJRINM4, respectively. (C) The production of β-Gal when cultured in LB supplemented with 5% maltose. The black square, white triangle, white circle, black diamond and white diamond represents the production of β-Gal from B. subtilis 1A747 harboring pJRINM1, pJRINM2, pJRINM3, pLJ-7 and pJRINM4; (D) SDS-PAGE analysis of β-Gal from the recombinants in B. subtilis 1A747 after 24 h cultured on a 12% SDS-polyacrylamide gel. β-Gal is indicated by the arrow. Molecular mass marker indicates (top to bottom): 116, 66, 45, 35 and 25 kDa.