Figure 3From: The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteinsIdentification of the intron of the HAC1 mRNA. A. Secondary mRNA structure of the 3'UTR of the HAC1 mRNA. The conserved nucleotides are highlighted in the figure. Conserved nucleotides are located in the distal end of the stem structure and are juxtaposed. B. Alignment of the 5' and 3' splice site of P. pastoris with the 5' and 3' border of the splice site of S. cerevisiae. Nucleotides in the box are present in the loop structure. C. New C-terminal domains are 18 amino acids in length and show conserved amino acids. D. Characteristic loop structures of the HAC1 mRNA of P. pastoris, S. cerevisiae and Human XBP-1. Predicted cleavage sites are indicated by an arrow, conserved nucleotides are boxed in grey.Back to article page