Table 3 Escherichia coli strains and plasmids used in this study.
Genotypes and relevant properties | Sources or references | |
|---|---|---|
Strains | ||
DH5α | F- end A1 gln V44 thi-1 rec A1 rel A1 gyr A96 deo R nup G ϕ80dlacZΔ M15 Δ(lacZYA-argF)U169, hsd R17(rK- mK+), λ- | Promega, USA |
BL21 | F- ompT gal dcm lon hsdS B (r B - m B -) | Novagen, USA |
BL21 (DE3) | F- ompT gal dcm lon hsdS B (r B - m B -) λ(DE3 [lac I lac UV5-T7 gene 1 ind 1 sam7 nin5]) | Novagen, USA |
Rosetta | F- ompT hsdS B (R B - m B -) gal dcm | Novagen, USA |
Rosetta (DE3) | F- ompT hsdS B (R B - m B -) gal dcm λ(DE3 [lac I lac UV5-T7 gene 1 ind 1 sam 7 nin 5]) | Novagen, USA |
Origami | F- ompT hsdSB rB- mB-) gal dcm lacY1 ahpC gor522::Tn10 (TcR) trxB::kan pAR5615 (ApR) | Novagen, USA |
Origami (DE3) | F- ompT hsdSB rB- mB-) gal dcm lacY1 ahpC gor522::Tn 10 (TcR) trxB::kan pAR5615 (ApR) λ(DE3[lac I lac UV5-T7 gene 1 ind 1 sam7 nin 5]) | Novagen, USA |
Plasmids | ||
pGEX-4T1 | AmpR, tac promoter, GST.Tag | GE HealthCare, USA |
pFO4 | AmpR, T7lac promoter, His.Tag | This Study |