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Figure 2 | Microbial Cell Factories

Figure 2

From: MARINE-EXPRESS: taking advantage of high throughput cloning and expression strategies for the post-genomic analysis of marine organisms

Figure 2

Assessing soluble and insoluble protein expression levels in the medium throughput strategy. a. SDS-PAGE of Insoluble (I) and soluble (S) crude cellular extracts of the E. coli expression cultures. Targets 50 to 60 are shown. Black circles surround bands of correct size for a given target. b. Western-blot using anti-His antibody of selected targets highlighted after purification of soluble cell lysis extracts on mini-affinity (His) columns. Selected targets between 156 and 181 are shown. Pink circles highlight bands of correct size for a given target.

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Microbial Cell Factories

ISSN: 1475-2859

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