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Table 2 GOX enzymatic activities.

From: Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus

Construction(a)

Final Biomassb

GOX activity(c)

  

Supernatant

Cell wall

Cell-bound

Total

 

(gDW/L)

U/mgProt

U/gDW

U/mgProt

U/gDW

U/mg Prot

U/gDW

U/gDW

Km1

7.4 ± 0.3

0.3 ± 0.1

0.5 ± 0.1

2.4 ± 0.1

1.9 ± 0.1

0.03 ± 0.02

4.0 ± 1.4

7.4 ± 2.5

Km2

12.1 ± 0.2

251 ± 72

407 ± 16

180 ± 24

1145 ± 35

1.3 ± 0.2

171 ± 44

1722 ± 62

Km3

12.0 ± 0.1

171 ± 44

255 ± 1

187 ± 8

850 ± 70

2.4 ± 0.3

291 ± 27

1395 ± 42

Kl1

9.9 ± 0.2

40 ± 3

161 ± 17

16 ± 1

13 ± 1

0.3 ± 0.1

31 ± 2

205 ± 16

Kl2

11.2 ± 0.0

32 ± 1

94 ± 6

64 ± 5

49 ± 8

0.1 ± 0.0

16 ± 3

158 ± 17

Kl3

9.6 ± 0.2

29 ± 1

94 ± 12

76 ± 4

64 ± 0

0.1 ± 0.0

13 ± 1

170 ± 11

  1. Heterologous GOX production in the supernatant, cell wall and cell-bound fractions of different K. marxianus and K. lactis strains.
  2. (a) see Materials and Methods section, Table 1 and Figure 1 for a description of the genetic constructions.
  3. (b) see Materials and Methods section, for a description of the cultivation conditions.
  4. (c) subcellular distribution according to Rouwenhorst et al. [50]
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Microbial Cell Factories

ISSN: 1475-2859

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