Skip to main content
Figure 6 | Microbial Cell Factories

Figure 6

From: Reduced evolvability of Escherichia coli MDS42, an IS-less cellular chassis for molecular and synthetic biology applications

Figure 6

Effect of ctxvp60 on cell growth. The graphs display growth curves of individual cultures, with the exception of panel E, where each line represents the median growth curve of 10 independent cultures. Cells were grown in LB at 37°C by inoculating colonies of freshly transformed cells grown on agar+Cam plates, with the exception of panel C, which displays cultures from panel A, re-inoculated into fresh medium at 1:100 volume. (A) pCTXVP60/MG1655, (B) pCTX/MG1655 (non-toxic control), (C) Re-inoculated pCTXVP60/MG1655 cultures from panel A, (D) pCTXVP60/MDS42, (E) pCTXVP60 in MDS cells with different number of resident ISes, indicated by the numbers, (F) pCTXVP60/MDS42+IS.

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us