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Table 3 Fermentation characteristics for S. cerevisiae wild-type strain CEN.PK 113-7D and the two mutants

From: Quantitative evaluation of yeast's requirement for glycerol formation in very high ethanol performance fed-batch process

 

WT

TEFmut7

TEFmut2

Fermentation Time (h)

55

49

49

Growth Time (h)

20

20

23

Final Volume (L)

3.10

2.89

2.92

μ max (h -1 )

0.35 ± 0.02

0.28 ± 0.02

0.24 ± 0.02

DCWmax (g L -1 )

15.7 ± 0.5

14.5 ± 0.5

14.5 ± 0.5

YDCW/glucose g g -1

0.093 ± 0.006 [5-15 h]

0.091 ± 0.007 [5-15 h]

0.087 ± 0.005 [5-17 h]

Yethanol/glucose g g -1 (growth)

0.41 ± 0.006 [0-20 h]

0.42 ± 0.01 [0-20 h]

0.44 ± 0.005 [0-23 h]

Yethanol/glucose g g -1 (overall)

0.43 ± 0.005 [0-55 h]

0.44 ± 0.008 [0-49 h]

0.45 ± 0.003 [0-49 h]

Y glycerol/glucose mg g -1

13 ± 0.01 [0-20 h]

5.1 ± 0.2 [0-20 h]

1.6 ± 0.1 [0-23 h]

[ethanol] final (g L -1 )

117 ±4

112 ± 4

114 ± 4

[glycerol] final (g L -1 )

3.1 ± 0.1

1 ± 0.1

0.4 ± 0.1

Ethanol Productivity (g L -1 h -1 )

2.13 ± 0.15

2.2 ± 0.15

2.3 ± 0.15

Pcritical/μ (g L -1 )

87 ± 3

85 ± 3

86 ± 3

  1. μmax: maximum specific growth rate; DCWmax: maximum cell concentration; Yi/glucose: production yield of the constituent i on glucose; Pcritical/μ: ethanol concentration at which growth stopped; "growth phase": fermentation phase of yeast growth; "production phase": fermentation phase after yeast growth stopped but ethanol production continued.
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Microbial Cell Factories

ISSN: 1475-2859

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