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Table 3 Fermentation characteristics for S. cerevisiae wild-type strain CEN.PK 113-7D and the two mutants

From: Quantitative evaluation of yeast's requirement for glycerol formation in very high ethanol performance fed-batch process

  WT TEFmut7 TEFmut2
Fermentation Time (h) 55 49 49
Growth Time (h) 20 20 23
Final Volume (L) 3.10 2.89 2.92
μ max (h -1 ) 0.35 ± 0.02 0.28 ± 0.02 0.24 ± 0.02
DCWmax (g L -1 ) 15.7 ± 0.5 14.5 ± 0.5 14.5 ± 0.5
YDCW/glucose g g -1 0.093 ± 0.006 [5-15 h] 0.091 ± 0.007 [5-15 h] 0.087 ± 0.005 [5-17 h]
Yethanol/glucose g g -1 (growth) 0.41 ± 0.006 [0-20 h] 0.42 ± 0.01 [0-20 h] 0.44 ± 0.005 [0-23 h]
Yethanol/glucose g g -1 (overall) 0.43 ± 0.005 [0-55 h] 0.44 ± 0.008 [0-49 h] 0.45 ± 0.003 [0-49 h]
Y glycerol/glucose mg g -1 13 ± 0.01 [0-20 h] 5.1 ± 0.2 [0-20 h] 1.6 ± 0.1 [0-23 h]
[ethanol] final (g L -1 ) 117 ±4 112 ± 4 114 ± 4
[glycerol] final (g L -1 ) 3.1 ± 0.1 1 ± 0.1 0.4 ± 0.1
Ethanol Productivity (g L -1 h -1 ) 2.13 ± 0.15 2.2 ± 0.15 2.3 ± 0.15
Pcritical/μ (g L -1 ) 87 ± 3 85 ± 3 86 ± 3
  1. μmax: maximum specific growth rate; DCWmax: maximum cell concentration; Yi/glucose: production yield of the constituent i on glucose; Pcritical/μ: ethanol concentration at which growth stopped; "growth phase": fermentation phase of yeast growth; "production phase": fermentation phase after yeast growth stopped but ethanol production continued.