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Figure 8 | Microbial Cell Factories

Figure 8

From: Novel approach of high cell density recombinant bioprocess development: Optimisation and scale-up from microlitre to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures

Figure 8

Growth curves of E. coli RV308/p ibpfxsT7lucA /pCTUT7MBP-RI cultures during the 6 × His-MBP-RI protein production process in a fed-batch bioreactor with exponential glucose feeding (A and B) and in a batch culture (C). Cultivations were performed at 37°C and induction was performed with 1 M IPTG. (A) Bioreactor process 1: fed-batch operation was started at OD600 = 6, induction at OD600 = 9 after the specific growth rate reached μ = 0.22 h-1. (B) Bioreactor process 2; fed-batch operation was started at OD600 = 22, induction at OD600 = 31 after the specific growth rate reached μ = 0.22 h-1. (C) Growth curve of the batch bioreactor process. Induction was performed at OD600 ≈ 5 at a specific growth rate of μ ≈ 0.45 h-1.

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Microbial Cell Factories

ISSN: 1475-2859

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