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Table 2 Characteristics of plasmids used in this study

From: Cocktail δ-integration: a novel method to construct cellulolytic enzyme expression ratio-optimized yeast strains

Plasmid

Recombination type

Selection marker

Expressing cellulase gene

pIHAGBGL-NotI

Integration

HIS3

β-Glucosidase

pIU-PGAGEG

 

URA3

Endoglucanase

pIW-PGAGCBH

 

TRP1

Cellobiohydrolase

pδW-PGAGBGL

δ-Integration

TRP1

β-Glucosidase

pδU-PGAGBGL

 

URA3

β-Glucosidase

pδH-PGAGBGL

 

HIS3

β-Glucosidase

pδW-PGAGEG

 

TRP1

Endoglucanase

pδU-PGAGEG

 

URA3

Endoglucanase

pδH-PGAGEG

 

HIS3

Endoglucanase

pδW-PGAGCBH

 

TRP1

Cellobiohydrolase

pδU-PGAGCBH

 

URA3

Cellobiohydrolase

pδH-PGAGCBH

 

HIS3

Cellobiohydrolase