Skip to main content

Table 1 Characteristics of bacterial and yeast strains used in this study

From: Cocktail δ-integration: a novel method to construct cellulolytic enzyme expression ratio-optimized yeast strains

Strain or plasmid

Relevant features

Reference

Bacterial strain

E. coli Novablue

endA1 hsdR17(r K12 -m K12 +) supE44 thi-I gyrA96 relA1 lac recA1/F [proAB + lacI q ZΔM15::Tn10(Tetr)]

Novagen

S. cerevisiae yeast strains

MT8-1

ade his3 leu2 trp1 ura3

[11]

MT8-1/IBEC

ade leu2 Integration of β-glucosidase, endoglucanase, and cellobiohydrolase gene

this study

MT8-1/δBEC

ade leu2 δ-Integration of β-glucosidase, endoglucanase, and cellobiohydrolase gene

this study

MT8-1/cocδBEC1

ade his3 leu2 ura3 cocktail δ-Integration of β-glucosidase, endoglucanase, and cellobiohydrolase gene

this study

MT8-1/cocδBEC2

ade his3 leu2 cocktail δ-Integration of β-glucosidase, endoglucanase, and cellobiohydrolase gene

this study

MT8-1/cocδBEC3

ade leu2 cocktail δ-Integration of β-glucosidase, endoglucanase, and cellobiohydrolase gene

this study