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Table 3 Strains and plasmids used and developed in this work.

From: Metabolic engineering for the production of shikimic acid in an evolved Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system

Strain/derivative

Relevant characteristics

Reference

E. coli JM101

supE, thi, Δ(lac-proAB), F'

[45]

E. coli JM101 aroK-

E. coli JM101 aroK Δ::cm

This work

E. coli aroB -

E. coli K12 strain BW25113 ΔaroB::kan (JW3352)

[48]

E. coli aroE -

E. coli K12 strain BW25113 ΔaroE::kan (JW3242)

[48]

E. coli PB28

PB12 ΔpykA::cat ΔpykF::gen

[31]

E. coli PB12

JM101 Δ(ptsH-I-crr)::kan glc+

[18]

PB12.SA1

PB12 ΔaroL

This work

PB12.SA11

PB12.SA1 pJLBaroGfbr tktA pTOPOaroB

This work

PB12.SA2

PB12 ΔaroL ΔaroK::cm

This work

PB12.SA21

PB12.SA2 pJLBaroGfbr tktA pTOPOaroB

This work

PB12.SA22

PB12.SA2 JLBaroGfbr tktA pTOPOaroB aroE

This work

PB12.SA3

PB12.SA2 ΔpykF::gen

This work

PB12.SA31

PB12.SA3 pJLBaroGfbr tktA pTOPOaroB aroE

This work

PB12.SA4

PB12.SA2 ΔpykA::gen

This work

PB12.SA41

PB12.SA4 pJLBaroGfbr tktA pTOPOaroB aroE

This work

E. coli TOP10

F- mcrA Δ(mrr-hsd RMS-mcrBC) φ 80lacZ ΔM15 ΔlacX 74 recA 1 ara D139 Δ(ara-leu)7697 galU galK rpsL end A1nupG

Invitrogen

Plasmids

  

pJLBaroGfbr tktA

pJLBaroGfbr (aroGfbr expressed from the lacUV5 promoter, lacI q and tet genes (Tcr), pACYC184 replication origin) derivative, containing the tktA gene with its native promoter.

[21, 47]

pCR®-Blunt II-TOPO®

Plac lacZ- α ORF T7 promoter ccdB kan (Kmr) Zeocin pUC origin.

Invitrogen

pTOPO aroB

pCR®-Blunt II-TOPO® containing the aroB gene

This work

pTOPO aroB aroE

pTOPOaroB derivative containing the aroB and aroE genes

This work