Figure 5

Effect of temperature on activity (A) and stability (B, C) of B. licheniformis mannan endo-1,4-β-mannosidase. The optimal temperature was determined using 0.5% LBG in 50 mM citrate buffer, pH 6.0 (A). The temperature stability was determined by measuring the remaining activity after incubation without substrate at various temperatures at pH 6.0 for 30 min, and measuring the residual activity using the standard assay (B). Panel C illustrates the remaining enzyme activity after incubation at 60°C (left) and 50°C (right) at various time points.