Table 1 Densitometric analysisaa
UreA source | Amount of proteins used (ng) | Density in OD/mm2 (standard deviation) | UreA concentration (ng) in extracts (% of total) | n° of recombinant proteins extracted from each spore |
|---|---|---|---|---|
Fusion 1 | ||||
Purified UreA | 6.25 ng | 38.9 (± 0.08) | NA | |
3.12 ng | 19,1 (± 0.11) | NA | ||
1.56 ng | 9.8 (± 0.06) | NA | ||
KH17 (CotB-UreA1) | 5.00 μg | 30,4 (± 0,03) | 4.93 (0.10) | |
2.50 μg | 15,1 (± 0,01) | 2.51 (0.10) | 1.1 × 10 3 | |
1.25 μg | 7,4 (± 0,02) | 1.12 (0.09) | ||
Fusion 2 | ||||
Purified UreA | 25.0 ng | 127.8 (± 0.02) | NA | |
12.5 ng | 63,3 (± 0.01) | NA | ||
6.25 ng | 32.1 (± 0.04) | NA | ||
KH10 (CotC-UreA1 wt background) | 2.50 μg | 99.1 (± 0.07) | 16.55 (0.66) | |
1.25 μg | 48.6 (± 0.09) | 8.14 (0.65) | 9.6 × 10 3 | |
0.625 μg | 23.6 (± 0.10) | 4.37 (0.70) | ||
KH11 (CotC-UreA1 cotC background) | 2.50 μg | 117.4 (± 0.03) | 20.82 (0.83) | |
1.25 μg | 57.7 (± 0.08) | 10.35 (0.83) | 15 × 10 3 | |
0.625 μg | 28.9 (± 0.21) | 5.15 (0.82) | ||
KH12 (CotC-UreA1 cotC cotU background) | 2.50 μg | 63.9 (± 0.03) | 11.10 (0.44) | |
1.25 μg | 30.7 (± 0.01) | 5.71 (0.46) | 7.2 × 10 3 | |
0.625 μg | 15.4 (± 0.03) | 2.93 (0.47) | ||
Fusion 3 | ||||
Purified UreA | 25.0 ng | 131.2 (± 0.30) | NA | |
12.5 ng | 64,1 (± 0.11) | NA | ||
6.25 ng | 31.6 (± 0.22) | NA | ||
KH23 (CotG-UreA cotG background) | 5.00 μg | 135,7 (± 0.25) | 28.42 (0.57) | |
2.50 μg | 68,2 (± 0.37) | 14.73 (0.59) | 5.3 × 10 3 | |
1.25 μg | 34,7 (± 0,05) | 7.15 (0.57) |