validation of the colorimetric assay for the VP-library. A) Linearity of the assay. Each point represents the average of 8 experiments (8 wells). B) Activities of VP-10C3 plotted in descending order. Dashed lines indicate the variation coefficient of the assay. S. cerevisiae cells were transformed with pJRoC30-VP-10C3 and plated on SC dropout plates. Individual colonies were picked and inoculated in a 96 well-plate. The activities of the clones were evaluated from fresh supernatant preparations.