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Table 1 Growth and pH characteristics in all cultivations. after induction) are presented.

From: A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures

Strain

Cultivation format

Medium

OD600 at ind.

pH at ind.

OD 600at harvest

pH at harvest

RB791 [pQE30:adh]

Ā 

LB

0.4

7.1

11.1

8.6

Ā 

24DWP

TB

0.6

7.0

12.7

8.4

Ā Ā 

MSM

0.2

7.2

17.5

6.8

Ā Ā 

Enbase Flo

9.3

7.0

51.3

6.9

BL21 (DE3) [pPal7:Dm_MFE-2]

Ā 

LB

0.6

7.2

5.9

8.0

Ā Ā 

TB

0.8

6.9

10.1

8.4

Ā 

24DWP

MSM

0.2

6.9

12.6

6.9

Ā Ā 

Enbase Flo

12.1

6.9

31.7

7.2

BL21(DE3) pLysS [pET23:pdi]

Ā 

LB

0.2

6.8

6.1

8.6

Ā Ā 

TB

0.5

7.0

16.9

8.5

Ā 

24DWP

MSM

0.2

7.2

2.5

6.8

Ā Ā 

Enbase Flo

12.1

7.0

33.3

7.3

Strain

Cultivation format

Medium

OD 600 at ind.

pH at ind.

OD 600 at harvest

pH at harvest

RB791 [pQE30:adh]

Ā 

LB

0.9

6.9

8.8

8.5

Ā 

shake flask

TB

0.7

7.3

17.1

8.3

Ā Ā 

MSM

0.3

7.2

9.8

5.8

Ā Ā 

Enbase Flo

9.2

6.9

32.2

7.4

BL21(DE3) [pPal7:Dm_MFE-2]

Ā 

LB

0.7

6.9

4.4

8.5

Ā 

shake flask

Enbase Flo

12.8

6.8

24.7

6.6

  1. OD600 and pH at induction and at final cell harvest (24 h