Table 1 GalE:α1,3-GalT fusion enzyme activity†
From: Metabolic engineering of Agrobacterium sp. strain ATCC 31749 for production of an α-Gal epitope
Strain | IPTG (mM) | Enzyme Activity [μmol/(min*L)] |
|---|---|---|
ATCC 31749/pBQET | 0 | 1.2 ± 0.76 |
0.05 | 10.7 ± 1.1 | |
0.1 | 28.0 ± 0.52 | |
0.5 | 53.4 ± 16.3 | |
1 | 54.1 ± 5.3 | |
ATCC 31749/pBQETY | 0 | 6.4 ± 0.96 |
0.05 | 13.2 ± 0.058 | |
0.1 | 15.6 ± 1.6 | |
0.5 | 14.3 ± 0.72 | |
1 | 11.6 ± 1.2 | |
ATCC 31749ΔcrdS/pBQET | 1 | 38.2 ± 1.7 |
ATCC 31749ΔcrdS/pBQETY | 1 | 12.6 ± 0.79 |