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Figure 4 | Microbial Cell Factories

Figure 4

From: Scale-up from microtiter plate to laboratory fermenter: evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentations

Figure 4

Maximum oxygen transfer capacity (OTR max ) and k L a-values versus the filling volume. for the chemical 0.5 mM sulfite system, the E. coli fermentation in WR medium and the Hansenula polymorpha fermentation in SYN6-MES medium. The method from Hermann et al. [9, 10] was applied for the determination and calculation of OTRmax and kLa-values for the sulfite system. The OTRmax and kLa-values for the fermentations were calculated by applying the proportionality factor (f) from table 1 on the OTRmax-values of the sulfite system. Operation conditions (Sulfite system/E. coli/H. polymorpha): various filling volumes between 100-260 μL, at 25°C/30°C/30°C temperature, 995 rpm shaking frequency and 3 mm shaking diameter.

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Microbial Cell Factories

ISSN: 1475-2859

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