Skip to main content

Table 4 Effect of detergents and enzyme inhibitors on esterase activity

From: Gene cloning and characterization of a novel esterase from activated sludge metagenome

Compounds

Concentration

Relative activity (%)

Control

0

100.0 ± 2.1

β-mercaptoethanl

1 mM

102.7 ± 2.7

DTT

1 mM

101.9 ± 1.9

SDS

1 mM

16.2 ± 9.3

Triton X-100

0.1%

119.6 ± 4.6

Tween 80

0.1%

128.9 ± 0.8

Tween 20

0.1%

135.8 ± 3.1

CTAB

1 mM

138.3 ± 2.1

PMSF

1 mM

100.3 ± 5.2

DEPC

1 mM

48.6 ± 0.7

  1. Activity without detergents and enzyme inhibitors was set as 100% (5290 U/ml). All measurements were done in triplicate.