Figure 3From: Gene cloning and characterization of a novel esterase from activated sludge metagenomeSDS-PAGE of overexpressed esterase EstAS in E. coli. Lane 1: molecular weight protein marker (Tiangen, Cat. No: MP102); lane 2, E. coli/pET28a: total protein extract, as negative control; lane 3: induced culture of E. coli/pEstAS-His, total protein extract; lane 4: purified EstAS (31 kDa).Back to article page