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Table 4 Biomass C-molecular formula calculated after the reconciliation procedure

From: Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels

 

C-mol Biomass formula

C:N

Ratio

H:O

Ratio

(γ)

Fab

Expressing

Strain

21% oxygen

C H1.665 N0.134 O0.602 S0.0039

7.45

2.77

4.11

 

11% oxygen

C H1.731 N0.137 O0.585 S0.0015

7.30

2.96

4.18

 

8% oxygen

C H1.775 N0.144 O0.579 S0.0012

6.94

3.06

4.20

Control

Strain

21% oxygen

C H1.761 N0.143 O0.636 S0.0018

7.02

2.77

4.09

 

11% oxygen

C H1.585 N0.135 O0.560 S0.0016

7.39

2.83

4.08

 

8% oxygen

C H1.726 N0.138 O0.581 S0.0014

7.26

2.97

4.17

S. cerevisiae *

C H1.748 N0.148 O0.596 S0.0018

6.76

2.93

4.22

  1. Elementary composition of biomas, expressed as C-molecular formula, for the different strains and experimental conditions (% O2 in the inlet air: 21, 11, 8). C:N carbon:nitrogen ratio; H:O hydrogen:oxygen ratio. γ: reduction degree of the biomass. * Data from Lange and Heijnen 2001 [11] for S. cerevisiae.