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Table 3 Biomass composition calculated after the reconciliation procedure

From: Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels

 

Fabb-expressing strain

Control strain

 

21%

 

11%

 

8%

 

21%

 

11%

 

8%

 
 

% w/w

sd

% w/w

sd

% w/w

sd

% w/w

sd

% w/w

sd

% w/w

sd

Protein

34.9

2.3

37.0

2.4

40.0

2.6

37.0

2.4

37.8

2.5

38.9

2.6

Carbohydrate

41.7

4.0

35.0

3.3

30.1

2.9

36.9

3.5

39.7

4.0

35.6

3.5

Lipid

6.6

3.4

9.8

3.2

10.3

3.2

6.2

3.3

7.8

3.5

8.7

3.3

RNA

6.9

0.7

6.2

0.6

6.1

0.6

6.6

0.7

6.0

0.6

4.9

0.5

DNA

0.14

0.01

0.15

0.01

0.15

0.01

0.13

0.01

0.13

0.01

0.13

0.01

SO4

0.2

0.2

0.2

0.2

0.1

0.2

0.3

0.3

2.7

3.5

0.2

0.2

H2O

3.2

2.5

5.2

2.4

6.8

2.2

6.3

2.4

0.2

0.2

5.0

2.4

Metals

6.4

0.4

6.4

0.4

6.4

0.4

6.4

0.4

5.6

1.2

6.5

0.4

C

44.3

1.4

44.6

1.4

44.4

1.4

43.0

1.4

45.6

1.2

44.7

1.4

H

6.2

0.2

6.5

0.2

6.6

0.2

6.3

0.2

6.0

0.2

6.4

0.2

N

6.7

0.4

6.9

0.4

7.4

0.4

6.9

0.4

7.0

0.4

7.0

0.4

O

35.6

1.4

34.8

1.4

34.3

1.4

36.4

1.4

34.1

1.3

34.6

1.4

S

0.2

0.1

0.2

0.1

0.1

0.1

0.2

0.1

0.2

0.1

0.2

0.1

Ashes

7.1

0.4

7.1

0.4

7.2

0.4

7.1

0.4

7.1

0.4

7.1

0.4

  1. Biomass composition in terms of macromolecular and elemental compounds for the different experimental conditions (% O2 in the inlet air 21, 11, 8).
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