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Table 1 Amino acid composition of the whole protein extract for all the conditions and strains tested

From: Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels

 

Pichia pastoris

 

% mol/mol

Fab-expressing Strain

Control strain

Saccharomyces cerevisiae *

 

21%

11%

8%

21%

11%

8%

 

Asx

8.82

9.78

9.59

8.78

9.51

9.78

9.28

Thr

5.88

5.98

5.78

5.77

5.90

6.16

5.57

Ser

6.26

6.65

6.73

6.43

6.76

6.90

5.33

Glx

17.81

16.24

15.64

18.55

16.19

14.33

15.48

Pro

3.83

4.08

4.47

3.67

4.00

4.76

4.22

Gly

6.86

7.34

7.42

7.12

7.28

7.53

8.89

Ala

10.40

9.92

10.13

10.68

9.74

9.88

9.77

Val

5.88

6.05

6.09

5.58

6.78

6.16

7.33

Cys

0.15

0.15

0.14

b.d.

0.22

0.13

0.14

Met

0.79

0.67

0.78

0.77

0.80

0.70

1.14

Ile

4.64

4.68

4.76

4.12

4.45

4.70

5.89

Leu

6.96

7.26

7.46

6.99

7.23

7.94

8.01

Tyr

2.16

2.22

2.27

2.13

2.21

2.47

1.96

Phe

3.20

3.30

3.33

3.03

3.21

3.37

3.76

Orn

1.04

1.02

0.86

1.53

1.21

0.93

0.24

Lys

6.41

7.07

6.93

6.33

7.03

6.93

6.57

His

1.89

1.87

1.87

1.79

1.78

1.89

1.93

Trp

1.40

1.40

1.40

1.40

1.40

1.40

1.96

Arg

7.04

5.72

5.76

6.74

5.68

5.45

3.86

  1. Amino acid composition measured for the different experimental conditions (% O2 in the inlet gas: 21, 11, 8) and for the different strains. Data given as percentage of each amino acid in the total pool of amino acids. * Data from Lange and Heijnen 2001 [11] for S. cerevisiae. Glx represents the pair Glu/Gln. Asx represents the pair Asp/Asn. b.d: below detection limit.
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Microbial Cell Factories

ISSN: 1475-2859

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